Function and regulation P-type ATPase-dependent lipid transport (II)

Many eukaryotic cells display an asymmetric distribution of phospholipids across their plasma membranes, yet its regulation and significance for the functioning of cells is poorly understood. Studies in yeast have implicated a novel subfamily of P-type ATPases in the transport of lipids across cellular membranes, and genome sequencing projects have revealed over a dozen of family members in eukaryotes but their biochemical functions are unclear. We will test whether members of this subfamily actually function as a membrane translocator of specific lipids by functional reconstitution into liposomes.